2012•Journal of Guizhou Normal UniversityRequires access

Determination of Puerarin in Health-care Food of Gezhi Capsules by HPLC

Fan Jiang

Abstract

Objective: To establish the determination method of puerarin in Gezhi capsules.Methods: HPLC method was used.The chromatographic column was Diamonsil C18(5μm,4.6mm×200 mm).The mobile phase was methanol-36% acetic acid-water(25∶3∶72).The flow rate was 0.8mL·min-1 and the detection wavelength was at 250nm.Results: The linear of puerarin was in the range of 0.063 68～0.700 5μg(r=0.999 9).The average recovery was 103.39% and RSD was 2.49%.Conclusion: The method has a good specificity,and it is accurate,reliable and reproducible.It can be used for the quality control of puerarin in Gezhi capsules.

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Available abstract

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetic acid, Linear relationship, Medicine