Effects of artemether on cell proliferation and cell cycle in SGC-7901 cells and ECV-304 cells in vitro

Aim:To investigate the effects of artemether(ART) on SGC-7901 cell proliferation in vitro.Methods:The effects of ART inhibiting cell proliferation in different concentration (1 000.00,500.00,250.00,125.00,62.50,and 31.25 mg/L) was determined by using improved Methy thiazolyl tetrazolium (MTT)assay. And human umbilical vein endothelial cell line was used as the corresponding frame of reference.The fluorescence flow cytometry(FCM)DNA assay was applied to detect the changes of apoptotic rate at early and late apopotosis process and cell proliferation and alteration of cell cycle phase.Results:With the time and doses of ART incubation extended,ART significantly inhibited the proliferation of SGC-7901 cells and the inhibitive effects showed dose-and time-dependent manner(P0.05).The IC50 value of SGC-7901 and ECV-304 cells was (504.74±3.87),(130.46±3.12),(83.46±2.92) and 1 000,1 000,(384.42±13.74) mg/L (P0.05). FCM assay indicated the apoptotic peak appeared. During the prolong of incubation time,the apoptosis rate was increased(P0.05).At the same time,the number of S and G2/M phase cells were decreased,and the cells were blocked in G0/G1 phase (P0.05).Conclusion:ART induces SGC-7901 cells apoptosis in vitro.