Effects of two-enzyme collaboration system on hydrolysis of soybean protein isolate

The influences of the ratio of neutral proteinase (1398) and alkaline proteinase (2709), enzymolysis pH, enzymolysis temperature and enzymolysis time on soybean protein enzymolysis was studied. The optimal conditions for double-enzyme hydrolysis were determined by orthogonal test and variance analysis. Single factor tests indicated that when the ratio of enzyme 1398 to 2709 was 1:3, the hydrolysis carried out very successfully at pH 9.5 and 60℃ for 3.5 h. The results of orthogonal test and ANOVA analysis indicated that the pH and the ratio of enzyme 1398 and 2709 had significant effects on hydrolysis (P0.05) .while the enzymolysis time and hydrolysis temperature did not have significant effects (P0.05).