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The Optimum of Enzymatic Hydrolysis Conditions of Soy Protein Using RSM

Yundong Mu

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Abstract

Bacillus subtilis As1.398 neutral proteinase was used to hydrolyze soybean protein isolate. Central composite design and Surface Analysis Methodology were applied to optimize the enzyme hydrolysis conditions of soybean protein isolate.Statistics Analysis System(SAS)was used to process the test results.The optimum hydrolysis conditions for Bacillus subtilis As1.398 neutral proteinase is temperature 40.2℃,pH7.2,the ratio of enzyme and substrate 0.87%,substrate concentration 8.86%, hydrolysis time 3 hours.The predict hydrolysis degree was 11.28% under these conditions and the test hydrolysis degree was 11.24%.

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What this paper is about

Bacillus subtilis As1.398 neutral proteinase was used to hydrolyze soybean protein isolate. Central composite design and Surface Analysis Methodology were applied to optimize the enzyme hydrolysis conditions of soybean protein isolate.Statistics Analysis System(SAS)was used to process the test results.The optimum hydrolysis conditions for Bacillus subtilis As1.398 neutral proteinase is temperature 40.2℃,pH7.2,the ratio of enzyme and substrate 0.87%,substrate concentration 8.86%, hydrolysis time 3 hours.The predict hydrolysis degree was 11.28% under these conditions and the test hydrolysis degree was 11.24%.

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Available abstract

Bacillus subtilis As1.398 neutral proteinase was used to hydrolyze soybean protein isolate. Central composite design and Surface Analysis Methodology were applied to optimize the enzyme hydrolysis conditions of soybean protein isolate.Statistics Analysis System(SAS)was used to process the test results.The optimum hydrolysis conditions for Bacillus subtilis As1.398 neutral proteinase is temperature 40.2℃,pH7.2,the ratio of enzyme and substrate 0.87%,substrate concentration 8.86%, hydrolysis time 3 hours.The predict hydrolysis degree was 11.28% under these conditions and the test hydrolysis degree was 11.24%.

Key concepts: Hydrolysis, Bacillus subtilis, Soy protein, Enzymatic hydrolysis, Response surface methodology, Chemistry, Substrate (aquarium), Enzyme

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