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2007•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Paeoniflorin in Fukang′an Soft Capsule by HPLC

Jia Heng

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Abstract

Aim To establish a method for determination of paeoniflorin in Fukang′an soft capsule. Methods The HPLC separation was performed on ZORBAX SB-C18(250mm×4.6mm,5μm) column with a mixture methanol-water(24∶76)as the mobile phase, at a flow rate of 1.0ml/min. Detection wavelength was at 230nm, and column temperature at 40℃. Results The linear response ranges from 0.033~0.330μg of paeoniflorin (r=0.999 9). The average recovery of a paeoniflorin was 98.63% with RSD 1.88%. Conclusion The method is simple, accurate, and suitable for the determination of paeoniflorin in compound preparations.

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What this paper is about

Aim To establish a method for determination of paeoniflorin in Fukang′an soft capsule. Methods The HPLC separation was performed on ZORBAX SB-C18(250mm×4.6mm,5μm) column with a mixture methanol-water(24∶76)as the mobile phase, at a flow rate of 1.0ml/min. Detection wavelength was at 230nm, and column temperature at 40℃. Results The linear response ranges from 0.033~0.330μg of paeoniflorin (r=0.999 9). The average recovery of a paeoniflorin was 98.63% with RSD 1.88%. Conclusion The method is simple, accurate, and suitable for the determination of paeoniflorin in compound preparations.

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Available abstract

Aim To establish a method for determination of paeoniflorin in Fukang′an soft capsule. Methods The HPLC separation was performed on ZORBAX SB-C18(250mm×4.6mm,5μm) column with a mixture methanol-water(24∶76)as the mobile phase, at a flow rate of 1.0ml/min. Detection wavelength was at 230nm, and column temperature at 40℃. Results The linear response ranges from 0.033~0.330μg of paeoniflorin (r=0.999 9). The average recovery of a paeoniflorin was 98.63% with RSD 1.88%. Conclusion The method is simple, accurate, and suitable for the determination of paeoniflorin in compound preparations.

Key concepts: Paeoniflorin, Chromatography, Capsule, High-performance liquid chromatography, Chemistry, Biology, Botany

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