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Determination of Paeoniflorin in Yushangping Capsule by HPLC

Chen Di-zhao

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Abstract

Objective:To establish method for the determination of Paeoniflorin in Yushangping Capsule.Methods:The separation was carried out on C18(250×4.6mm,5μm)column;the mobile phase was composed of acetonitrile-0.1%H3PO4(17∶83),detection wavelength was 230nm.Results:The linear range of Paeoniflorin was 0.2112~2.1120μg(r=0.9999);average recovery was 99.36%(RSD=1.96%,n=9).Conclusion:This method was accurate,rapid,reproducible and suitable for the quality control of this preparation.

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What this paper is about

Objective:To establish method for the determination of Paeoniflorin in Yushangping Capsule.Methods:The separation was carried out on C18(250×4.6mm,5μm)column;the mobile phase was composed of acetonitrile-0.1%H3PO4(17∶83),detection wavelength was 230nm.Results:The linear range of Paeoniflorin was 0.2112~2.1120μg(r=0.9999);average recovery was 99.36%(RSD=1.96%,n=9).Conclusion:This method was accurate,rapid,reproducible and suitable for the quality control of this preparation.

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Available abstract

Objective:To establish method for the determination of Paeoniflorin in Yushangping Capsule.Methods:The separation was carried out on C18(250×4.6mm,5μm)column;the mobile phase was composed of acetonitrile-0.1%H3PO4(17∶83),detection wavelength was 230nm.Results:The linear range of Paeoniflorin was 0.2112~2.1120μg(r=0.9999);average recovery was 99.36%(RSD=1.96%,n=9).Conclusion:This method was accurate,rapid,reproducible and suitable for the quality control of this preparation.

Key concepts: Paeoniflorin, Chromatography, Capsule, High-performance liquid chromatography, Linear range, Chemistry, Detection limit, Biology

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