ScholarLens Back to search
2012•Dali Xueyuan xuebaoRequires access

Determination of Chlorogenic Acid Content in Xiaoerjinqiao Granules by RP-HPLC

Yongshou Yang

Open publisher page 0 citations

Abstract

Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules. Methods: We applied the Agilent XBP-C18(4.6 mm×250 mm,5μm) as the chromatographic column,and the mobile phase with acetonitrile-0.4% phosphoric acid solution(12:88) at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm,and the column temperature of 25 ℃.Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL(r =1.0). The average recovery was 100.44% and RSD was 1.50%.Conclusion: The method appears to be simple,accurate and reliable,and can be used for the quality control of Xiaoerjinqiao Granules.

About this research paper

What this paper is about

Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules. Methods: We applied the Agilent XBP-C18(4.6 mm×250 mm,5μm) as the chromatographic column,and the mobile phase with acetonitrile-0.4% phosphoric acid solution(12:88) at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm,and the column temperature of 25 ℃.Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL(r =1.0). The average recovery was 100.44% and RSD was 1.50%.Conclusion: The method appears to be simple,accurate and reliable,and can be used for the quality control of Xiaoerjinqiao Granules.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules. Methods: We applied the Agilent XBP-C18(4.6 mm×250 mm,5μm) as the chromatographic column,and the mobile phase with acetonitrile-0.4% phosphoric acid solution(12:88) at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm,and the column temperature of 25 ℃.Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL(r =1.0). The average recovery was 100.44% and RSD was 1.50%.Conclusion: The method appears to be simple,accurate and reliable,and can be used for the quality control of Xiaoerjinqiao Granules.

Key concepts: Chromatography, Chlorogenic acid, Phosphoric acid, High-performance liquid chromatography, Content determination, Chemistry, Linear relationship, Acetonitrile

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Chlorogenic Acid Content in Xiaoerjinqiao Granules by RP-HPLC — Research Paper | ScholarLens