2014•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Chlorogenic Acid in Anti-inflammatory Granules by RP-HPLC

Wang Jun-ji

Abstract

Objective To establish an HPLC method for the determination of chlorogenic acid in anti-inflammatory granules. Methods C18column( 150 mm × 4. 6 mm,5 μm) was used. The mobile phase consisted of 0. 1% phosphoric acid solution-acetonitrile( 9∶ 91) at a flow rate of 1. 0 ml·min- 1. The temperature of the column was 30 ℃ and the detection wavelength was 327 nm. Results There was a good linear relationship within the range of 8. 128- 60. 96 μg·ml- 1( r =0. 9998). The average recovery rate was 99. 34%,with an RSD of 0. 41%( n = 9). Conclusion This method is quick,accurate,sensitive,repeatable and can be used for quality control of anti-inflammatory granules.

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Key concepts: Chlorogenic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship, Acetonitrile