ScholarLens Back to search
2008•Zhongguo xin yao zazhiRequires access

Determination of chlorogenic acid in Gankeqing granules by HPLC

Shuzhen Zhang

Open publisher page 0 citations

Abstract

Objective:To establish an HPLC method for the determination of chlorogenic acid in Gankeqing granules.Methods:An HPLC method was carried on a Kromasil C18 column(150 mm×4.6 mm,5 μm),the mobile phase was cetonitrile-0.4% phosphoric acid(10∶90),the flow rate was 1.0 mL·min-1,the detecting wavelength was set at 327 nm.Results:The linearity was good within a range of 0.15~0.75 μg(r= 0.999 6).The average recovery was 98.77%,RSD=1.30%(n=6).Conclusion:This method is rapid,simple and accurate,and can be used for the quality control of Gankeqing granules.

About this research paper

What this paper is about

Objective:To establish an HPLC method for the determination of chlorogenic acid in Gankeqing granules.Methods:An HPLC method was carried on a Kromasil C18 column(150 mm×4.6 mm,5 μm),the mobile phase was cetonitrile-0.4% phosphoric acid(10∶90),the flow rate was 1.0 mL·min-1,the detecting wavelength was set at 327 nm.Results:The linearity was good within a range of 0.15~0.75 μg(r= 0.999 6).The average recovery was 98.77%,RSD=1.30%(n=6).Conclusion:This method is rapid,simple and accurate,and can be used for the quality control of Gankeqing granules.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish an HPLC method for the determination of chlorogenic acid in Gankeqing granules.Methods:An HPLC method was carried on a Kromasil C18 column(150 mm×4.6 mm,5 μm),the mobile phase was cetonitrile-0.4% phosphoric acid(10∶90),the flow rate was 1.0 mL·min-1,the detecting wavelength was set at 327 nm.Results:The linearity was good within a range of 0.15~0.75 μg(r= 0.999 6).The average recovery was 98.77%,RSD=1.30%(n=6).Conclusion:This method is rapid,simple and accurate,and can be used for the quality control of Gankeqing granules.

Key concepts: Chromatography, Chlorogenic acid, Phosphoric acid, High-performance liquid chromatography, Chemistry, Organic chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of chlorogenic acid in Gankeqing granules by HPLC — Research Paper | ScholarLens