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Study of Cell Growth Inhibition and Apoptosis Induced by Oxaliplatin in Human Lung Adenocarcinoma Cell Line-A549

Guo Zheng-li

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Abstract

Objective To research in vitro chemosensitivity and apoptosis induced by oxaliplatin in human lung adenocarcinoma cell line-A549. Methods A549 was incubated with oxaliplatin of virous concentration , cell growth inhibition was assesed by MTT colorimetric assay and apoptotic rate was detected by FCM. Results At dose of 100,50,25,12.5 μg/ml, oxaliplation apparently inhibited growth of A549 cell, FACS can showed apoptotic rate was 35%,13.6%,2.6%,1.4% for 24 h treatment at dose of 100,50,25,12.5 μg/ml respectively. Conclusion The cell growth inhibition of oxaliplatin on A549 is dose-dependent and time-dependent, oxaliplatin can induce apoptosis in A549 cell which is dose-dependent. There is a positive linear correlation between apoptosis assessed by FACS can and growth inhibition rates by MTT( P 0.01).

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Objective To research in vitro chemosensitivity and apoptosis induced by oxaliplatin in human lung adenocarcinoma cell line-A549. Methods A549 was incubated with oxaliplatin of virous concentration , cell growth inhibition was assesed by MTT colorimetric assay and apoptotic rate was detected by FCM. Results At dose of 100,50,25,12.5 μg/ml, oxaliplation apparently inhibited growth of A549 cell, FACS can showed apoptotic rate was 35%,13.6%,2.6%,1.4% for 24 h treatment at dose of 100,50,25,12.5 μg/ml respectively. Conclusion The cell growth inhibition of oxaliplatin on A549 is dose-dependent and time-dependent, oxaliplatin can induce apoptosis in A549 cell which is dose-dependent. There is a positive linear correlation between apoptosis assessed by FACS can and growth inhibition rates by MTT( P 0.01).

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Available abstract

Objective To research in vitro chemosensitivity and apoptosis induced by oxaliplatin in human lung adenocarcinoma cell line-A549. Methods A549 was incubated with oxaliplatin of virous concentration , cell growth inhibition was assesed by MTT colorimetric assay and apoptotic rate was detected by FCM. Results At dose of 100,50,25,12.5 μg/ml, oxaliplation apparently inhibited growth of A549 cell, FACS can showed apoptotic rate was 35%,13.6%,2.6%,1.4% for 24 h treatment at dose of 100,50,25,12.5 μg/ml respectively. Conclusion The cell growth inhibition of oxaliplatin on A549 is dose-dependent and time-dependent, oxaliplatin can induce apoptosis in A549 cell which is dose-dependent. There is a positive linear correlation between apoptosis assessed by FACS can and growth inhibition rates by MTT( P 0.01).

Key concepts: Oxaliplatin, A549 cell, Apoptosis, Cell growth, MTT assay, Cell culture, Adenocarcinoma, Growth inhibition

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