Study of cell growth inhibition and apoptosis induced by oxaliplatin in human poor differentiation nasopharyngeal carcinoma cell line-CNE2

Background and purpose:Backgrounds and Objective: Oxaliplatin is a third-generation platinum compound,there are some distinct differeuces in mechanism between cisplatin and oxaliplatin,and oxaliplatin is less toxic Poorly differentiated cartinoma is the most common histological type,for nasopharyngeal carcinoma,although radiotherapy is the basic therapeutic approach to nasopharyngeal carcinoma,chemotherapy is also important for recurrent and metastatic nasopharyngeal carcinoma,so in this work we analyzed the effects of oxaliplatin on nasopharyngeal carcinoma cell lines in vitro.Methods:CNE2 was incubated with oxaliplatin at various concentrations and times,cell growth inhibition was assessed by MTT-microculture tetrazolium assay,cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopy transmission electron.Results:Growth of CNE2 cells was significantly inhibited in a dose-dependent and time-dependment fashion.The inhibition of cell growth inhibition was(95.6±0.7)% after incubation with 100 ug/ml oxaliplatin for 48 hours,the cell was arrested at G_(2)/M and apoptosis was induced.When CNE2 cells were treated with oxaliplatin at the concentration of 0、0.03、4.0、100 μg/ml,the rates of CNE2 cell apoptosis were(0.19±0.17)% 、(0.37±0.09)%、(5.50±1.08)%、(9.43±0.09)%,respectively.24 hours after 20 μg/ml the CNE2 cells were characterized by chromatin condensation,chromatin crescent formation,nucleus fragmentation and apoptosis body by transmission electron microscopy.Conclusions:Oxaliplatin suppresses the growth of CNE2 cells in vitro by causing cell-cycle arrest and cell apoptosis.