Antifungal Activity of Ethanol Extract from Veratrum Linn

In order to discover the agricultural activity and fungicidal ingredient in the Veratrum Linn.and provide scientific proofs of its exploitation and utilization,antifungal activity of the extracts from Veratrum Linn.with ethanol and different solvents were studied.Using a mycelial growth method,the fungistasis of the extracts with ethanol against six agricultural fungi were investigated in this study.The results showed that the extracts had strong antifungal activity against the tested pathogenic fungi.The inhibition rate was 96.4%,78.1% and 74.3% with the concentration of 5.0mg/mL against Valsa mali,Fusarium oxysporum f.sp.vasinfectum and Physalospora piricola nose,respectively.The ethanol extract was fractionated using solvents of diffrent polarity,the ethyl acetate fraction and n-butanol fraction had stronger antifungal activities than that of extracts by solvents water.The EC50 of ethyl acetate and n-butanol fraction against V.mali was 0.161mg/ml and 0.190 mg/ml,respectively.The EC50 of ethyl acetate and n-butanol fraction against Physalospora piricola nose was 0.551mg/ml and 0.451 mg/ml,respectively.The ethyl acetate fraction could be acted as the emphases for further study.