Isolation and Identification of Antifungal Components from Juniperus chinensis cv.Kaizuca with Ethyl Acetate
Hao Ming-liang
Abstract
Hao Ming-liang
Abstract
Antifungal components,which isolated from ethanol extract of Juniperus chinensis cv.Kaizuca with ethyl acetate,were tested against Valsa mali,Coniothyrium diplodiella,and so on,based on bioassay-guided method.The results showed that ethyl acetate extracts of J.chinensis had certain inhibition effects to all tested fungi,and the best inhibition rates against V.mali and C.diplodiella were 83.06% and 78.87% at 2 mg/mL,respectively.The EC50 of the active component Savinin against V.mali,C.diplodiella,Gibberella zeae,Elsinoe ampelina,Physalospora piricola and Fusarium oxysporum were 0.38,0.18,0.47,0.59,0.70 mg/mL,and 1.94 mg/mL,respectively.
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Antifungal components,which isolated from ethanol extract of Juniperus chinensis cv.Kaizuca with ethyl acetate,were tested against Valsa mali,Coniothyrium diplodiella,and so on,based on bioassay-guided method.The results showed that ethyl acetate extracts of J.chinensis had certain inhibition effects to all tested fungi,and the best inhibition rates against V.mali and C.diplodiella were 83.06% and 78.87% at 2 mg/mL,respectively.The EC50 of the active component Savinin against V.mali,C.diplodiella,Gibberella zeae,Elsinoe ampelina,Physalospora piricola and Fusarium oxysporum were 0.38,0.18,0.47,0.59,0.70 mg/mL,and 1.94 mg/mL,respectively.
Key concepts: Ethyl acetate, Fusarium oxysporum, EC50, Bioassay, Gibberella, Gibberella zeae, Antifungal, Botany