Antifungal activity of ethanol extrtacts from Peganum harmala L.

The hypha growth-inhibiting method and the virulence determination method were used to investigate the inhibition effects of the petroleum ether fraction,chloroform fraction,ethyl acetate fraction,n-butanol fraction and water fraction in the alcoholic extracts from the aboveground of Peganum harmala L.on the five types of popular plant pathogenic fungi,Alternaria solani,Phytohthora capsici,Fusarium bulbigenum,Fusarium oxysporum f.niveum and Valsa mali.The results showed that chloroform fraction,n-butanol fraction,and ethylacetate fraction can inhibit the growth of five types of popular plant pathogenic fungi.The inhibition effect of chloroform fraction was the most potent,with the inhibition rate about more than 83 to 5 plant pathogenic fungi under the concentration of 15 mg/mL.Under the concentration of 15 mg/mL,the ethyl acetate fraction and n-butanol fraction thoroughly inhibited the growth of Alternaria solani and Valsa mali,with the inhibition rate up to 100.The n-butanol fraction showed the highest potent virulence,with EC_ 50 to Alternaria solani and Valsa mali about 0.09 and 0.279 mg/mL respectively.