Study on Antifungal Activity of Several Plant Pathogenit Fungi From Ethanol Extract of Luffa

The antifungal activity constituents were extracted fromLuffawith ethanol,and the extract were separated by petroleum ether,ethylacetate,n-butanol and water.The antifungal activity was determined to ten plant pathogens by mycelial growth inhibition assay.The results showed that the ethanol extracts of Luffahad strong inhibition effect against Glomerella cingulata,Penicillium expansum,Physalospora piricola and Fusarium oxysporium,their inhibitory rate came up to 73.5%,72.2%,66.8%and 61.1%at 5.00×103 mg/L.The highest activity components were distributed in petroleum ether and ethyl acetate partition.The EC50 value of petroleum ether partition against Glomerella cingulata and Penicillium expansum were 3.2×103 mg/L and 3.9×103 mg/L;the EC50 value of ethyl acetate partition were 1.2×103mg/L and 2.1×103 mg/L,respectively.The petroleum ether and ethyl acetate partition was the most active partition and could be acted as the emphases for further study.