Determination of nicotine in edible fungi by ultra performance liquid chromatography-tandem mass spectrometry

An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS)method was established for the determination of nicotine in edible fungi.The samples were extracted by water with ultrasonic wave.The separation of nicotine was performed on Waters Acquity UPLC system with a BEH C18 column(50 mm×2.1 mm,1.7 μm) and the mobile phase was composed of 50%acetonitrile and 50%water(containing 0.1% ethanoic acid) at a flow rate of 0.4 mL/min and nicotine was determined by positive ESI MS/MS.The parent ion of positive ESI MS was m/z 163.2,and the daughter ion of MS/MS was m/z 130.1.The method was quantified by external standard method.The calibration curve was good linear in the concentration range of 0.0001～0.050 mg/L(r=0.9999).The limit of detection was 0.001 mg/kg.The recoveries of nicotine were in the range of 97.2%～98.5% and the RSD was less than 2.0%.The method was proved to be simple and accurate.