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Simultaneous Determination of 7 Kinds of β_2-agonist in Feed by UPLC-MS/MS

Yan Ke-xu

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Abstract

A method of ultra performance liquid chromatograghy-electrospray tandem mass spectrometry(UPLC-MS/MS) was studied for determination of β2-agonist in feed.The sample was extracted by 0.1 mol/L hydrochloric acid-methanol mixed solvent,purified and enriched with cationic solid phase extraction column,and which was separated by liquid chromatography on C18 column with mobile phase of 0.1% formic acid in methanol(gradient elution),then analyzed in UPLC-MS/MS with electrospray ion source(ESI) under multi-reaction monitor mode(MRM).The quantification was performed with external standard method.The method has a lower limit of quantitation 50 μg/kg.Spike recoveries of three concentration addition levels(50,100,200 μg/kg) ranged in 71%—103% with relative standard deviations in 5.7%—9.8%.The method is fast,accurate and sensitive.It is suitable for rapid qualitative and quantitative detection of β2-agonist in animal feed.

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What this paper is about

A method of ultra performance liquid chromatograghy-electrospray tandem mass spectrometry(UPLC-MS/MS) was studied for determination of β2-agonist in feed.The sample was extracted by 0.1 mol/L hydrochloric acid-methanol mixed solvent,purified and enriched with cationic solid phase extraction column,and which was separated by liquid chromatography on C18 column with mobile phase of 0.1% formic acid in methanol(gradient elution),then analyzed in UPLC-MS/MS with electrospray ion source(ESI) under multi-reaction monitor mode(MRM).The quantification was performed with external standard method.The method has a lower limit of quantitation 50 μg/kg.Spike recoveries of three concentration addition levels(50,100,200 μg/kg) ranged in 71%—103% with relative standard deviations in 5.7%—9.8%.The method is fast,accurate and sensitive.It is suitable for rapid qualitative and quantitative detection of β2-agonist in animal feed.

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Available abstract

A method of ultra performance liquid chromatograghy-electrospray tandem mass spectrometry(UPLC-MS/MS) was studied for determination of β2-agonist in feed.The sample was extracted by 0.1 mol/L hydrochloric acid-methanol mixed solvent,purified and enriched with cationic solid phase extraction column,and which was separated by liquid chromatography on C18 column with mobile phase of 0.1% formic acid in methanol(gradient elution),then analyzed in UPLC-MS/MS with electrospray ion source(ESI) under multi-reaction monitor mode(MRM).The quantification was performed with external standard method.The method has a lower limit of quantitation 50 μg/kg.Spike recoveries of three concentration addition levels(50,100,200 μg/kg) ranged in 71%—103% with relative standard deviations in 5.7%—9.8%.The method is fast,accurate and sensitive.It is suitable for rapid qualitative and quantitative detection of β2-agonist in animal feed.

Key concepts: Chemistry, Chromatography, Formic acid, Selected reaction monitoring, Electrospray, Extraction (chemistry), Detection limit, High-performance liquid chromatography

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Simultaneous Determination of 7 Kinds of β_2-agonist in Feed by UPLC-MS/MS — Research Paper | ScholarLens