Expression and Significance of Midkine mRNA in Oral Carcinoma

Objective:To study the expression and significance of midkine(MK)mRNA in oral carcinoma.Methods:RT-PCR and in situ hybridization analysis for MK mRNA were performed on 50 cases of oral carcinoma(20 cases with transfer lymph nodes,30 cases of benign tissue and 30 cases of normal tissue).Results:RT-PCR half-quantity analysis:there were obviously different expressions between cancer and lymph node tissues,and the quantity of the former had exceeded the latter(P0.01).43 of 50 cancer cases expressed MK.1 of 30 benign tumors expressed MK,and normal tissues didn't express MK,and tMK only expressed in cancer.MK mRNA positive products(-brown-yellow grains)lied in the cytoplasm of cancer cells,cell nucleus occasionally.Benign tumors expressed faintly and normal tissues didn't express or faintly.The quantity of MK mRNA in cancer was higher than the control group(P0.01).There was statistic significance between metastasic group and non-metastasic group(P0.05).The expression of MK mRNA increased with clinical stage,especially most of Ⅲ and Ⅳ A stage cases with lymph nodes metastatic expressed mid-strong,strong positive.The result of RT-PCR was the same as the result of in situ hybridization(P0.05).Conclusion:MK mRNA was overexpressed in oral carcinoma,and was correlated with malignancy degree of disease.tMK might be a marker of malignancy tumor.