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2002•Zhonghua shiyan waike zazhiRequires access

Increased midkine mRNA expression in hepatocellular carcinoma

Zongdi Wu

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Abstract

Objective To investigate the expression of midkine (MK) mRNA in hepatocellular carcinoma (PHCC) and its clinical implications.Methods Liver tissues were obtained from 33 patients with HCC,10 with benign liver tumors and 5 with normal controls.Total RNA was extracted from tissues by using TRIzol method.MK mRNA expression was detected by using RT PCR.Results MK mRNA expression was detectable in the concerous tissues of 28 HCC patients (84.8%) and in the tissues adjacent to the concers of 3 cases.All the benign liver tumors and normal tissues were negative for MK mRNA expression (P0.01).No significant difference was found in clinical and pathological parameters between MK mRNA negative and positive cases.Conclusion MK is expressed at mRNA level in the majority of HCC tissues and rarely in the adjacent tissues.MK might be used as a marker for HCC.

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Objective To investigate the expression of midkine (MK) mRNA in hepatocellular carcinoma (PHCC) and its clinical implications.Methods Liver tissues were obtained from 33 patients with HCC,10 with benign liver tumors and 5 with normal controls.Total RNA was extracted from tissues by using TRIzol method.MK mRNA expression was detected by using RT PCR.Results MK mRNA expression was detectable in the concerous tissues of 28 HCC patients (84.8%) and in the tissues adjacent to the concers of 3 cases.All the benign liver tumors and normal tissues were negative for MK mRNA expression (P0.01).No significant difference was found in clinical and pathological parameters between MK mRNA negative and positive cases.Conclusion MK is expressed at mRNA level in the majority of HCC tissues and rarely in the adjacent tissues.MK might be used as a marker for HCC.

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Available abstract

Objective To investigate the expression of midkine (MK) mRNA in hepatocellular carcinoma (PHCC) and its clinical implications.Methods Liver tissues were obtained from 33 patients with HCC,10 with benign liver tumors and 5 with normal controls.Total RNA was extracted from tissues by using TRIzol method.MK mRNA expression was detected by using RT PCR.Results MK mRNA expression was detectable in the concerous tissues of 28 HCC patients (84.8%) and in the tissues adjacent to the concers of 3 cases.All the benign liver tumors and normal tissues were negative for MK mRNA expression (P0.01).No significant difference was found in clinical and pathological parameters between MK mRNA negative and positive cases.Conclusion MK is expressed at mRNA level in the majority of HCC tissues and rarely in the adjacent tissues.MK might be used as a marker for HCC.

Key concepts: Midkine, Hepatocellular carcinoma, Messenger RNA, Trizol, Pathological, Pathology, RNA, Medicine

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