Study for Association between the Angiogenesis II Type I Receptor Gene Polymorphism and Essential Hypertension in Hans of Ningxia

Objective:To investigate the relationship between the polymorphism of angiontensin Ⅱ type 1 receptor(AT1R)gene A1166C and essential hypertension(EH)in Hans in Ningxia Province,China.Methods: The genomic DNAs were extracted from the peripheral blood of 336 peoples in Ningxia Region.Polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) methods were used to detect the A1166C polymorphism of AT1R gene in Hans including 160 patients with EH and 176 normal controls.The frequencies of genotype distribution in EH and normal groups were studied.Results: The distribution genotype frequencies of AA,AC and CC were 93.1%,6.3% and 0.6% in hypertension groups,respectively,and those in normal groups were 87.5%,11.4% and 1.1%,respectively,which showed no significant difference(P0.05);The allelic frequencies of A and C were 96.3% and 3.8%,respectively in hypertension group,while 93.2% and 6.8% in the normal group.The allele frequencies of A/ C were no significant differences to above data in two groups(P0.05).Conclusions: It is no significant association found between the A1166C polymorphism of AT1R gene and essential hypertension in Hans of Ningxia.