Effect of Shugan Hewei Decoction on the Expression of Induced Nitric Oxide Synthase,5-hydroxytryptamine and substance P in Gastric Antrum of Functional Dyspepsia Rats

Objective To observe the effect of Shugan Hewei Decoction(SHD) on the expression of induced nitric oxide synthase(iNOS),5-hydroxytryptamine(5-HT) and substance P(SP) in gastric antrum functional dyspepsia rats.Methods The rats were divided into 5 groups randomly: the normal saline group,model group,mosapride group,the large,middle and small dosage of SHD groups.Rats were treated after irritated furious by clamping their tails for making functional dyspepsia of TCM type of disharmony between liver and stomach.Gastric antrum tissue was sampled for immunohistochemistry for observing the effects.After the rats were treated,the iNOS,5-HT and SP in gastric antrum were detected by the methods.Results SHD can reduce the expression of iNOS in model gastric antrum tissue significantly,and improve the expression of 5-HT and SP(P0.05,P0.01),and all showed insignificant difference in comparison to mosapride group.Conclusion The function of lowering the expression of iNOS and improving the expression of 5-HT and SP in the gastric antrum tissue may be one of its mechanisms.