2012•Guide of China MedicineRequires access

HPLC Determination of Five the Cypress Parameters Lotion Matrine Content

Qiong Liu

Abstract

Objective To establish a method for the content determination of matrine in Wubaishen lotion.Methods HPLC method was used for the content determination.Eclipse XDB-C18 column was used with mobile phase consisted of acetonitrile-phosphate buffer(PH 6.8,V/V=25∶75)at the flow rate of 1.0mL/min.The injection volume was 10μL.The detection wavelength was set at 210 nm and the column temperature was 30℃.Results Matrine showed a good linear relationship within the range of 27.00~135.00mg/mL(r=0.999 5)and the average recoveries were 98.28%,RSD=1.85%(n=9).Conclusion The method is accurate,sensitive and reproducible.It can be used for the content determination of matrine and the quality control of Wubaishen lotion.

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Key concepts: Matrine, Lotion, Content determination, Chromatography, Medicine, High-performance liquid chromatography, Traditional medicine, Chemistry