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Determination of matrine content in Yinyanjing lotion by HPLC

WU Ya-li

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Abstract

Objective To establish an HPLC method for the determination of matrine in Yinyanjing lotion.Methods The column of VP-ODS(5 μm,150 mm×4.6 mm)was used.The mobile phase was methanol-water(10:90,containing 0.4% triethylamine solution,pH adjusted to 6.9 with phosphoric acid).The flow rate was 1 mL·min-1,the detection wavelength was 220 nm;and the column temperature was 42 ℃.Results There was good linear relationship for matrine at 0.04~0.24 mg·mL-1,r=0.999 8.RSD=0.93%.The average recovery was 99.94%,RSD=0.53%(n=9).Conclusion The method is simple,accurate,and reproducible.It can be used in the regular analysis and the quality control of matrine in Yinyanjing lotion.

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What this paper is about

Objective To establish an HPLC method for the determination of matrine in Yinyanjing lotion.Methods The column of VP-ODS(5 μm,150 mm×4.6 mm)was used.The mobile phase was methanol-water(10:90,containing 0.4% triethylamine solution,pH adjusted to 6.9 with phosphoric acid).The flow rate was 1 mL·min-1,the detection wavelength was 220 nm;and the column temperature was 42 ℃.Results There was good linear relationship for matrine at 0.04~0.24 mg·mL-1,r=0.999 8.RSD=0.93%.The average recovery was 99.94%,RSD=0.53%(n=9).Conclusion The method is simple,accurate,and reproducible.It can be used in the regular analysis and the quality control of matrine in Yinyanjing lotion.

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Available abstract

Objective To establish an HPLC method for the determination of matrine in Yinyanjing lotion.Methods The column of VP-ODS(5 μm,150 mm×4.6 mm)was used.The mobile phase was methanol-water(10:90,containing 0.4% triethylamine solution,pH adjusted to 6.9 with phosphoric acid).The flow rate was 1 mL·min-1,the detection wavelength was 220 nm;and the column temperature was 42 ℃.Results There was good linear relationship for matrine at 0.04~0.24 mg·mL-1,r=0.999 8.RSD=0.93%.The average recovery was 99.94%,RSD=0.53%(n=9).Conclusion The method is simple,accurate,and reproducible.It can be used in the regular analysis and the quality control of matrine in Yinyanjing lotion.

Key concepts: Matrine, Lotion, Chromatography, Chemistry, Phosphoric acid, Content determination, High-performance liquid chromatography, Triethylamine

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