Determination of Quercetin,Kaempferol and Isorhamnetin in Different Seabuckthorn Leaves by HPLC

Objective To determine the contents of three major flavonoids in different seabuckthorn leaves.Methods The contents of quercetin,kaempferol and isorhamnetin were analyzed simultaneously by HPLC on a Shim-pack VP-ODS column(4.6 mm×150 mm,5μm)at temperature 30 ℃.The mobile phase was consisted of methanol and 0.4% phosphoric acid(50∶50),with flow rate of 0.4mL·min-1,and the detection wavelength was set at 370 nm.Results The linear ranges of quercetin,kaempferol and isorhamnetin were 1.67-106.60μg/mL(r=0.999 9),0.51-32.64μg/mL(r=0.999 6),0.78-49.92μg/mL(r=0.999 9),and their average recoveries were 99.50%,98.00% and 100.80% respectively.There were significant differences in flavonoids contents among different seabuckthorn leaves.Conclusion The HPLC method is simple,accurate and applicable for the quality evaluation of seabuckthorn leaves.