Determination of Four Kinds of Flavonoids in Lotus Leaves

To establish a quick and easy method of qualitative and quantitative analysis for determining the content of hyperoside,quercetin,kaempferol and isorhamnetin in lotus leaves by HPLC.Results:Under conditions of Gemini C18 for the chromatographic column and 40℃ for column temperature,with methanol-0.1% phosphoric aicd(55∶45,v∶v)of the mobile phase and 1mL/min of flow velocity,360 nm for the detection wavelength,the linear ranges of hyperoside,quercetin,kaempferol and isorhamnetin were 1.5~48.0μg/mL,1.0~32.0μg/mL,1.0~32.0μg/mL,0.7~24.0μg/mL,respectively,the recovery was 90%~103%and the precision was 3.5%~4.2%,the contents of four kinds of flavonoids in lotus leaves were respectively 1.94%,0.092%,0.025% and 0.021%.The proposed method was suitable for the determination of hyperoside,quercetin,kaempferol and isorhamnetin in lotus leaves.