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2010•Liaoning Zhongyiyao Daxue xuebaoRequires access

Determ Ination of Quercetin and Isorhamnetin in Seabuckthorn by HPLC

Yanli Yang

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Abstract

Objective:To establish the method of detecting the contents of quercetin and isorhamnetin,and to detectire the contents of the two types of aglycone in difforont parts of seabuckthorn.Methods :The mobile phase consisted of Methanol-0.4% phosphoric aicd(58 :42).The detective wavelength was at 370nm.Detective the hydrlyed sample which was after methanol extract.Results :The contents of quercetin and isorhamnetin in the stems,leares and fruit of seabuckthorn wore respectively:0.276,2.11,0.574,0.463,1.60,0.729mg/g.Conclusion :This method is simple,quick and accurate.

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What this paper is about

Objective:To establish the method of detecting the contents of quercetin and isorhamnetin,and to detectire the contents of the two types of aglycone in difforont parts of seabuckthorn.Methods :The mobile phase consisted of Methanol-0.4% phosphoric aicd(58 :42).The detective wavelength was at 370nm.Detective the hydrlyed sample which was after methanol extract.Results :The contents of quercetin and isorhamnetin in the stems,leares and fruit of seabuckthorn wore respectively:0.276,2.11,0.574,0.463,1.60,0.729mg/g.Conclusion :This method is simple,quick and accurate.

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Available abstract

Objective:To establish the method of detecting the contents of quercetin and isorhamnetin,and to detectire the contents of the two types of aglycone in difforont parts of seabuckthorn.Methods :The mobile phase consisted of Methanol-0.4% phosphoric aicd(58 :42).The detective wavelength was at 370nm.Detective the hydrlyed sample which was after methanol extract.Results :The contents of quercetin and isorhamnetin in the stems,leares and fruit of seabuckthorn wore respectively:0.276,2.11,0.574,0.463,1.60,0.729mg/g.Conclusion :This method is simple,quick and accurate.

Key concepts: Isorhamnetin, Quercetin, Aglycone, Medicine, Traditional medicine, Methanol, Chromatography, Kaempferol

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Determ Ination of Quercetin and Isorhamnetin in Seabuckthorn by HPLC — Research Paper | ScholarLens