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2013•Strait Pharmaceutical JournalRequires access

Determination of Icariin in Bushen Huoxue Tablets by HPLC

Xin Yi-cun

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Abstract

OBJECTIVE To establish a high performance liquid chromatographic(HPLC) method for the determination of icariin in Bushen Huoxue Tablets.METHODS The HPLC determination was carried out with Agilent ZORBAX SB-C18 column(250mm × 4.6mm,5μm).The mobile phase was consisted of acetonitrile-0.1% phosphoric acid(25∶ 75) with the flow rate of 1.0mL.min-1,and the detection wavelength was 270nm.RESULTS Icariin showed a good linearity in the range 16.16μg.mL-1 ～ 323.2μg.mL-1,the coefficient was 0.9997,and the average recovery was 99.66%(RSD = 0.78%).CONCLUSION The method is simple and accurate with good reproducibility,and can be used for the quality control of Bushen Huoxue Tablets.

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What this paper is about

OBJECTIVE To establish a high performance liquid chromatographic(HPLC) method for the determination of icariin in Bushen Huoxue Tablets.METHODS The HPLC determination was carried out with Agilent ZORBAX SB-C18 column(250mm × 4.6mm,5μm).The mobile phase was consisted of acetonitrile-0.1% phosphoric acid(25∶ 75) with the flow rate of 1.0mL.min-1,and the detection wavelength was 270nm.RESULTS Icariin showed a good linearity in the range 16.16μg.mL-1 ～ 323.2μg.mL-1,the coefficient was 0.9997,and the average recovery was 99.66%(RSD = 0.78%).CONCLUSION The method is simple and accurate with good reproducibility,and can be used for the quality control of Bushen Huoxue Tablets.

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Available abstract

OBJECTIVE To establish a high performance liquid chromatographic(HPLC) method for the determination of icariin in Bushen Huoxue Tablets.METHODS The HPLC determination was carried out with Agilent ZORBAX SB-C18 column(250mm × 4.6mm,5μm).The mobile phase was consisted of acetonitrile-0.1% phosphoric acid(25∶ 75) with the flow rate of 1.0mL.min-1,and the detection wavelength was 270nm.RESULTS Icariin showed a good linearity in the range 16.16μg.mL-1 ～ 323.2μg.mL-1,the coefficient was 0.9997,and the average recovery was 99.66%(RSD = 0.78%).CONCLUSION The method is simple and accurate with good reproducibility,and can be used for the quality control of Bushen Huoxue Tablets.

Key concepts: Icariin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Reproducibility, Content determination, Medicine

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