1996•Europe PMC (PubMed Central)Requires access

Upland Rice Transformation

Zhihua Yu, Chen Minyong, GU Hong-ya, Pan Nai-sui, Zhangliang Chen, Yan Li

Abstract

Herbicide resistant transgenic upland rice plants were recovered using biolistic method. Upland rice suspension cells and immature embryos were transformed by particle bombardment with a plasmid containing herbicide resistant bar gene. One week after bombardment, the bombarded cells were transferred to N6 medium with phosphinothricin (PPT) for selection. Fertile transgenic plants were regenerated from PPT resistant calli. Integration of bar gene was confirmed by Southern blot hybridization. Resistance of R0 transgenic plants to herbicide PPT was shown by Basta (commercial form of PPT) applications in the greenhouse. Inheritance of bar gene was demonstrated by PCR analysis of R1 transgenic plants.

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Key concepts: Biology, Southern blot, Transformation (genetics), Genetically modified rice, Genetically modified crops, Gene, Transgene, Botany