Studies on transferring herbicide resistance bar gene into wheat by particle bombardment

The plant expression vector carrying the herbicide resistance bar gene was introduced into wheat immature embryo calli using particle bombardment.Parameters of bombardment were optimized by GUS transient expression,such as precipitants,distance,and amount of gold powder.After selection on media supplemented with different concentration of PPT,33 resistance plants were regenerated.It was demonstrated that the bar gene had been intergraded into 4 plants genome and expressed in transgenic plants by PCR analysis and spaying herbicide on the leaf.The average transformation frequency is about 0.33%.