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Amplification and identification of dendritic cells from mouse bone marrow

Junbo Xia, Kui Wu, Kun Sun, Changzheng Wang

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Abstract

Objective To improve the methods for in vitro generating dendritic cells (DCs) from mouse bone marrow and to identify it with morphological, phenotype determination. Methods Cells isolated from mice bone marrow were cultured in GM-CSF (20 ng/ml), differentiating into dendritic cells. Morphological changes were observed by optical phase contrast microscopy, and surface molecules including CD11 C, CD80, MHCⅡ were detected by FACS. Results A large number of typical DCs were observed after culturing for 10 d. FACS analysis showed that the amplified DCs could express CD11 C, CD80, MHCⅡ. Conclusion A large quantity of highly pure BM-DC can be obtained by this method.

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What this paper is about

Objective To improve the methods for in vitro generating dendritic cells (DCs) from mouse bone marrow and to identify it with morphological, phenotype determination. Methods Cells isolated from mice bone marrow were cultured in GM-CSF (20 ng/ml), differentiating into dendritic cells. Morphological changes were observed by optical phase contrast microscopy, and surface molecules including CD11 C, CD80, MHCⅡ were detected by FACS. Results A large number of typical DCs were observed after culturing for 10 d. FACS analysis showed that the amplified DCs could express CD11 C, CD80, MHCⅡ. Conclusion A large quantity of highly pure BM-DC can be obtained by this method.

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Available abstract

Objective To improve the methods for in vitro generating dendritic cells (DCs) from mouse bone marrow and to identify it with morphological, phenotype determination. Methods Cells isolated from mice bone marrow were cultured in GM-CSF (20 ng/ml), differentiating into dendritic cells. Morphological changes were observed by optical phase contrast microscopy, and surface molecules including CD11 C, CD80, MHCⅡ were detected by FACS. Results A large number of typical DCs were observed after culturing for 10 d. FACS analysis showed that the amplified DCs could express CD11 C, CD80, MHCⅡ. Conclusion A large quantity of highly pure BM-DC can be obtained by this method.

Key concepts: Bone marrow, CD80, Molecular biology, Follicular dendritic cells, Dendritic cell, Major histocompatibility complex, Phenotype, Cell biology

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