2013•China Animal Husbandry & Veterinary MedicineRequires access
Isolation and Culture of Mouse Bone Marrow-derived Dendritic Cells
Guangle Jia
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Abstract
To establish a method of isolation,cultivation and purification of dendritic cells from mouse bone marrow in vitro.Mononuclear cells isolated form mouse bone marrow were induced into immature dendritic cells by being cultured with rGM-CSF and then examined from aspects of morphology,phenotype and function of T lymphocyte proliferation.The result showed that a large number of typical dendritic cells were observed after culturing for 9 days,and high expressed CD11c 81.09%,middle exproessed MHCⅡ,low expressed CD40,CD80,CD86.A method to generate large number of DCs from mouse bone marrow in vitro had been established.