ScholarLens Back to search
2009•Zhonghua zhongliu fangzhi zazhiRequires access

Expanding dendritic cells derived from mouse bone-marrow in vitro

Jin Huang

Open publisher page 0 citations

Abstract

OBJECTIVE:To establish a method of expanding dendritic cells from mouse bone marrow in vitro.METHODS: Mononuclear cells isolated from mouse bone marrow were induced into dendritic cells by being cultured in GM-CSF and IL-4,and then examined from their morphology,phenotype and function.RESULTS: A large number of morphologically typical dendritic cells were observed after the culturing for 8 days,and high expressed CD11c(83.19%),MHC-Ⅱ(95.25%),and CD86(89.24%),and their capacity to stimulate primary T cell responses in the mixed leukocyte reaction(MLR) was shown strongly.CONCLUSION: A large number of dendritic cells can be generated by culturing the mononuclear cells derived from mouse bone marrow in vitro,establishing a foundation for producing the anti-tumour vaccine in next step.

About this research paper

What this paper is about

OBJECTIVE:To establish a method of expanding dendritic cells from mouse bone marrow in vitro.METHODS: Mononuclear cells isolated from mouse bone marrow were induced into dendritic cells by being cultured in GM-CSF and IL-4,and then examined from their morphology,phenotype and function.RESULTS: A large number of morphologically typical dendritic cells were observed after the culturing for 8 days,and high expressed CD11c(83.19%),MHC-Ⅱ(95.25%),and CD86(89.24%),and their capacity to stimulate primary T cell responses in the mixed leukocyte reaction(MLR) was shown strongly.CONCLUSION: A large number of dendritic cells can be generated by culturing the mononuclear cells derived from mouse bone marrow in vitro,establishing a foundation for producing the anti-tumour vaccine in next step.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE:To establish a method of expanding dendritic cells from mouse bone marrow in vitro.METHODS: Mononuclear cells isolated from mouse bone marrow were induced into dendritic cells by being cultured in GM-CSF and IL-4,and then examined from their morphology,phenotype and function.RESULTS: A large number of morphologically typical dendritic cells were observed after the culturing for 8 days,and high expressed CD11c(83.19%),MHC-Ⅱ(95.25%),and CD86(89.24%),and their capacity to stimulate primary T cell responses in the mixed leukocyte reaction(MLR) was shown strongly.CONCLUSION: A large number of dendritic cells can be generated by culturing the mononuclear cells derived from mouse bone marrow in vitro,establishing a foundation for producing the anti-tumour vaccine in next step.

Key concepts: Bone marrow, CD11c, Follicular dendritic cells, Dendritic cell, In vitro, Peripheral blood mononuclear cell, Mixed lymphocyte reaction, CD86

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Expanding dendritic cells derived from mouse bone-marrow in vitro — Research Paper | ScholarLens