2004•Chinese Journal of Current Advances in General SurgeryRequires access
Construction of eukaryotic expression plasmid encoding full-length angiostatin from Kunming mouse strain
Bo Tang
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Abstract
Objective:To construct the eukaryotic expression plasmid encoding full-length angiostatin from Kunming mouse strain. Methods:The angiostatin cDNA was amplified from the tRNA isolated from Kunming mouse liver tissues by RT-PCR. Subsequently ,the PCR product was cloned into pGEMT vector and subcloned into eukaryotic expression plasmid pcDNA3.1(+) at HindⅢ and XbaⅠcut sites.Results:The sequencing results showed that the angiostatin gene has two variations in nucleotide sequence and one amino acid residue displaces, compared with the published sequence.Conclusion:The eukaryotic expression vector, pcDNA3.1(+)-angiostatin, has been successfully constructed, which may have potential value in cancer anti-angiogenesis therapy.