ScholarLens Back to search
2003•Academic Journal of Kaifeng Medical CollegeRequires access

Cloning of Mouse Angiostatin and Construction of its Eukaryotic Expression Vector

Xiujuan Li

Open publisher page 0 citations

Abstract

Objective: To clone the sequence of mouse Angiostatin cDNA and construct its eukaryotic expression vector, provide a base for its antitumor effect. Methods: According to the sequence of Mouse angiostatin cDNA in Genebank, Mouse angiostatin cDNA was amplified by RT-PCR and ligated into pMD18T vector. Through the vector pKS, the pCMV-Angiostatin recombinant plasmid was constructed. Results: The sequence of mouse Angiostatin cDNA is identical with reported. Angiostatin cDNA was inserted into expression vector correctly. Conclusion: Mouse Angiostatin cDNA is cloned and pCMV-Angiostatin recombinant plasmid is completed successfully.

About this research paper

What this paper is about

Objective: To clone the sequence of mouse Angiostatin cDNA and construct its eukaryotic expression vector, provide a base for its antitumor effect. Methods: According to the sequence of Mouse angiostatin cDNA in Genebank, Mouse angiostatin cDNA was amplified by RT-PCR and ligated into pMD18T vector. Through the vector pKS, the pCMV-Angiostatin recombinant plasmid was constructed. Results: The sequence of mouse Angiostatin cDNA is identical with reported. Angiostatin cDNA was inserted into expression vector correctly. Conclusion: Mouse Angiostatin cDNA is cloned and pCMV-Angiostatin recombinant plasmid is completed successfully.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective: To clone the sequence of mouse Angiostatin cDNA and construct its eukaryotic expression vector, provide a base for its antitumor effect. Methods: According to the sequence of Mouse angiostatin cDNA in Genebank, Mouse angiostatin cDNA was amplified by RT-PCR and ligated into pMD18T vector. Through the vector pKS, the pCMV-Angiostatin recombinant plasmid was constructed. Results: The sequence of mouse Angiostatin cDNA is identical with reported. Angiostatin cDNA was inserted into expression vector correctly. Conclusion: Mouse Angiostatin cDNA is cloned and pCMV-Angiostatin recombinant plasmid is completed successfully.

Key concepts: Angiostatin, Complementary DNA, Recombinant DNA, Biology, Molecular biology, Plasmid, Expression vector, Cloning (programming)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning of Mouse Angiostatin and Construction of its Eukaryotic Expression Vector — Research Paper | ScholarLens