Cloning of Mouse Interleukin-18 and Construction of Eukaryotic Expression Plasmid

The cDNA of mouse interleukin-18(IL-18) was cloned and recombined with plasmid pcDNA3.1/His B to construct an eukaryotic expression plasmid.The cDNA of mouse IL-18 was amplified from the liver cells by RTPCR.Having being digested by Hind III and EcoR I,this cDNA fragment was inserted into expression vector pcDNA3.1/His B.The recombinant plasmid was identified by restriction enzyme digest,PCR and sequencing.The identification has confirmed that the eukaryotic expression plasmid pcDNA3.1/ mIL-18 had been successfully constructed,which has provided foundation for further research.