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2005•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of chlorogenic acid in Kanggan granules by HPLC

Shou Wen-hong

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Abstract

OBJECTIVE To establish a HPLC method for determination of chlorogenic acid in Kanggan granules.METHOD A ZORBAX SB-C_ 18 column (4.6mm×250mm,5μm) was used with a mobile phase of methanol-1% phosphoric acid solution (25∶75).The detect wavelength was 327 nm.RESULTS The calibration curve was linear within the range of 7.5～120.0μg/mL (r=0.9999),and the average recovery of chlorogenic acid was 100.48%(RSD=1.2%).CONCLUSION The method is simple,rapid and accurate. It can be used for quantitative analysis of Kanggan granules.

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What this paper is about

OBJECTIVE To establish a HPLC method for determination of chlorogenic acid in Kanggan granules.METHOD A ZORBAX SB-C_ 18 column (4.6mm×250mm,5μm) was used with a mobile phase of methanol-1% phosphoric acid solution (25∶75).The detect wavelength was 327 nm.RESULTS The calibration curve was linear within the range of 7.5～120.0μg/mL (r=0.9999),and the average recovery of chlorogenic acid was 100.48%(RSD=1.2%).CONCLUSION The method is simple,rapid and accurate. It can be used for quantitative analysis of Kanggan granules.

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Available abstract

OBJECTIVE To establish a HPLC method for determination of chlorogenic acid in Kanggan granules.METHOD A ZORBAX SB-C_ 18 column (4.6mm×250mm,5μm) was used with a mobile phase of methanol-1% phosphoric acid solution (25∶75).The detect wavelength was 327 nm.RESULTS The calibration curve was linear within the range of 7.5～120.0μg/mL (r=0.9999),and the average recovery of chlorogenic acid was 100.48%(RSD=1.2%).CONCLUSION The method is simple,rapid and accurate. It can be used for quantitative analysis of Kanggan granules.

Key concepts: Chlorogenic acid, Phosphoric acid, Calibration curve, Chromatography, High-performance liquid chromatography, Chemistry, Methanol, Linear range

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