Cloning and functional identification of the root specific promoter from corn

【Objective】 This paper aimed to clone and identify the root-specific promoter of ZmGLU1P gene from corn.【Method】 The promoter region of root-specific gene was isolated from the genomic DNA of corn P138 by PCR method,and obtained the promoter fragment ZmGLU1P.The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pCAMBIA121-ZmGLU1P,which was transferred into tobacco(Nicotiana tabacum) NC89 by Agrobacteriumtum efaciens-mediated method and several transformed plants were obtained.Then they were detected by PCR and Southern blotting.The root,stem,and leaf of gram-positive recombinant corns were performed with GUS activity assay.【Result】 The results showed that the length of the clone was 1 846 bp,and the sequence was more than 99% homology compared with the reported promoters.PCR and Southern blot results showed that the transgenic plants were obtained successfully.GUS activity assays of different organs indicated that the expression of GUS was active mostly in roots,suggesting that the ZmGLU1P gene is root-specific promoter.【Conclusion】 The cloned 1846bp fragment β-glucosidase gene from corn is root-specific promoter.