Simultaneous Determination of Icariin and Naringin in Bushen Huayu Extract by HPLC

Objective To establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC. Methods Reverse-phase high performance liquid chromatography( RP-HPLC) separation was performed one C18column(4. 6 mm × 250 mm,5 μm). The mobile phase was acetonitrile containing 0. 2% H3PO4(pH =2.9).Gradient elution with a flow rate of 0. 8 mL·min- 1was applied to achieve the separation. The detection wavelength was set at269 nm,and the column temperature was 30 ℃. Results Icariin had a good linear range from 0. 3- 3. 0 μg(r = 0. 999 9).The recovery rate was 105. 6%,and RSD was 0. 95%. Naringin was linear within the range of 0. 12- 1. 20 μg( r = 0. 999 9).The recovery rate was 94. 0% and RSD was 0. 52%. Conclusion The method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.