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2010•Shanxi Yike Daxue xuebaoRequires access

Determination of Icariin in Tian’e bushen oral liquid by HPLC

Yun Kong

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Abstract

Objective To establish a HPLC method for determining the contents of Icariin in Tian’e bushen oral liquid. Methods Hypersil BDS column was used with acetonitrile-phosphoric acid(35∶65) as the mobile phase,at the detective wavelength of 270 nm and column temperature of 25 ℃. Results There was a good linear relationship for Icariin in the range of 0.201 0-1.004 8 μg,Y=804 362X+144 60(r=0.999 9) .The average recovery was 99.8%,and RSD was 1.03%. Conclusion The method is simple,accurate and repeatable,and can be used to control the quality of Tian’e bushen oral liquid.

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What this paper is about

Objective To establish a HPLC method for determining the contents of Icariin in Tian’e bushen oral liquid. Methods Hypersil BDS column was used with acetonitrile-phosphoric acid(35∶65) as the mobile phase,at the detective wavelength of 270 nm and column temperature of 25 ℃. Results There was a good linear relationship for Icariin in the range of 0.201 0-1.004 8 μg,Y=804 362X+144 60(r=0.999 9) .The average recovery was 99.8%,and RSD was 1.03%. Conclusion The method is simple,accurate and repeatable,and can be used to control the quality of Tian’e bushen oral liquid.

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Available abstract

Objective To establish a HPLC method for determining the contents of Icariin in Tian’e bushen oral liquid. Methods Hypersil BDS column was used with acetonitrile-phosphoric acid(35∶65) as the mobile phase,at the detective wavelength of 270 nm and column temperature of 25 ℃. Results There was a good linear relationship for Icariin in the range of 0.201 0-1.004 8 μg,Y=804 362X+144 60(r=0.999 9) .The average recovery was 99.8%,and RSD was 1.03%. Conclusion The method is simple,accurate and repeatable,and can be used to control the quality of Tian’e bushen oral liquid.

Key concepts: Icariin, High-performance liquid chromatography, Chromatography, Phosphoric acid, Tian, Chemistry, Linear relationship, Medicine

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