Callus Induction and Plantlet Regeneration from Mature Embryoes of Festuca arundinacea

[Objective] The study was to optimize the tissue culture conditions of Festuca arundinacea mature embryoes.[Methods] With F.arundinacea mature embryos as the explants and MS as the basic medium.The effects of different concentrations of hormones and their combinations on callus induction,subculture and plant differentiation were studied.[Results] The callus induction rate of the explants in MS medium with 2,4-D 2.0 mg/L was the highest,reaching 63.5%.In the range of 1.0-4.0 mg/L,with the decrease of 2,4-D concentration in the medium the graininess and compactness of the callus increased,and its water content decreased.The optimum subculture medium for callus was MS +2,4-D 2.0 mg/L.The plant differentiation rate of callus in MS medium with 2.0 mg/L 6-BA and 0.10 mg/L NAA was the highest,reaching 80.2%.The regenerated plants were transplanted into 1/2 MS medium without plant growth regulator for proliferation and strong seedling,and then they were transplanted into sandy loam and their transplantation survival rate could reach 90%.[Conclusion] This study established the efficient regeneration system of F.arundinacea.