The establishment of high-freguency plant regeneration System of Festuca arundiance

Aim To establish a high-freqency regeneration system of tall fescue in order to supply a better receptor system for transformation of relevant genes.Methods Use mature seeds of fescue arundinacea and MS medium were respectively as explants and basical medium to perfume embryonic callus induction,subculture,differentiation and rooting medium.Results After somatic embryos formationon MS medium with 2,4-D 5.0 mg/L,CH 400 mg/L,sucrose 60 g/L and agar 12 g/L,shoots were regenerated on MS medium containing 6-BA 2.0 mg/L+NAA 0.5 mg/L.The ratio of callus differentiation reached higher level of 78.9%.Regenerated shoots were rooted on half strength MS medium supplemented with 0.5 mg/L NAA,rate of rooting reaching 100%.Conclusion MS medium containing 2,4-D 9.0 mg/L was optimal for callus induction;MS medium containing 6-BA 2.0 mg/L+NAA 0.5 mg/L was optimal for differentiateon;Half strength MS medium supplemented with 0.5 mg/L NAA was optmal for rooting.