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2015•Journal of Sichuan UniversityRequires access

Genetic diversity analysis of Rhododendron(Ericaceae)located on Jinfo mountain

Zhong Ron

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Abstract

Genetic relationship of four species in Rhododendron on Chongqing Jinfo Mountain was preliminary analyzed using inter-simple sequence repeat(ISSR)marker.Thirteen primers were screened out from 100 random primers and a total of 239 DNA fragments(about 171 were polymorphic)were amplified from 200~1500bp.The average number of DNA fragments produce by each primer was 18.38.The Nei'S genetic distances were calculated by using DPS software and the dendrogram were constructed by UPGMA method in NTSYSpc2.1.The results revealed that these 4strains of Rhododendron have a similar genetic background with certain differences.And the four Rhododendronspecies were obviously divided into two groups:R.changii was clustered into one group:R.pauciflorum,R.jingfuense and R.platypodum were clustered into the other.

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What this paper is about

Genetic relationship of four species in Rhododendron on Chongqing Jinfo Mountain was preliminary analyzed using inter-simple sequence repeat(ISSR)marker.Thirteen primers were screened out from 100 random primers and a total of 239 DNA fragments(about 171 were polymorphic)were amplified from 200~1500bp.The average number of DNA fragments produce by each primer was 18.38.The Nei'S genetic distances were calculated by using DPS software and the dendrogram were constructed by UPGMA method in NTSYSpc2.1.The results revealed that these 4strains of Rhododendron have a similar genetic background with certain differences.And the four Rhododendronspecies were obviously divided into two groups:R.changii was clustered into one group:R.pauciflorum,R.jingfuense and R.platypodum were clustered into the other.

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Available abstract

Genetic relationship of four species in Rhododendron on Chongqing Jinfo Mountain was preliminary analyzed using inter-simple sequence repeat(ISSR)marker.Thirteen primers were screened out from 100 random primers and a total of 239 DNA fragments(about 171 were polymorphic)were amplified from 200~1500bp.The average number of DNA fragments produce by each primer was 18.38.The Nei'S genetic distances were calculated by using DPS software and the dendrogram were constructed by UPGMA method in NTSYSpc2.1.The results revealed that these 4strains of Rhododendron have a similar genetic background with certain differences.And the four Rhododendronspecies were obviously divided into two groups:R.changii was clustered into one group:R.pauciflorum,R.jingfuense and R.platypodum were clustered into the other.

Key concepts: UPGMA, Ericaceae, Dendrogram, Genetic diversity, Biology, Primer (cosmetics), Botany, Genetic variation

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