ScholarLens Back to search
2009•Lishizhen Medicine and Materia Medica ResearchRequires access

Genetic Diversity of Dendrobium Species (Orchidaceae) Plants in China by ISSR

Tao Liu

Open publisher page 2 citations

Abstract

Objective To probe the relationship among Dendrobium species(Orchidaceae).Methods 14 primers with good polymorphism screened from 55 ISSR primers were used to conduct ISSR analysis and dimorphic data of DNA fragments were used to construct the dendrogram by UPGMA.Results Totally 123 bands were amplified and 112 of them were polymorphic accounting for 91.06%.The average number of DNA bands amplified by each primer was 8.79.Nei's gene diversity(H),Shannon's information index(I) and effective number of alleles(NE) were 0.351 9,0.528 2 and 1.594 5,respectively.The genetic similarity among all the tested clonal cultivars ranged from 0.090 9 to 0.695 7,averaging 0.377 9,which indicated that the genetic basis was relatively rich.Conclusion Clustering results revealed that the dendrogram constructed with ISSR molecular makers was identical with the classic taxonomic system.

About this research paper

What this paper is about

Objective To probe the relationship among Dendrobium species(Orchidaceae).Methods 14 primers with good polymorphism screened from 55 ISSR primers were used to conduct ISSR analysis and dimorphic data of DNA fragments were used to construct the dendrogram by UPGMA.Results Totally 123 bands were amplified and 112 of them were polymorphic accounting for 91.06%.The average number of DNA bands amplified by each primer was 8.79.Nei's gene diversity(H),Shannon's information index(I) and effective number of alleles(NE) were 0.351 9,0.528 2 and 1.594 5,respectively.The genetic similarity among all the tested clonal cultivars ranged from 0.090 9 to 0.695 7,averaging 0.377 9,which indicated that the genetic basis was relatively rich.Conclusion Clustering results revealed that the dendrogram constructed with ISSR molecular makers was identical with the classic taxonomic system.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To probe the relationship among Dendrobium species(Orchidaceae).Methods 14 primers with good polymorphism screened from 55 ISSR primers were used to conduct ISSR analysis and dimorphic data of DNA fragments were used to construct the dendrogram by UPGMA.Results Totally 123 bands were amplified and 112 of them were polymorphic accounting for 91.06%.The average number of DNA bands amplified by each primer was 8.79.Nei's gene diversity(H),Shannon's information index(I) and effective number of alleles(NE) were 0.351 9,0.528 2 and 1.594 5,respectively.The genetic similarity among all the tested clonal cultivars ranged from 0.090 9 to 0.695 7,averaging 0.377 9,which indicated that the genetic basis was relatively rich.Conclusion Clustering results revealed that the dendrogram constructed with ISSR molecular makers was identical with the classic taxonomic system.

Key concepts: UPGMA, Dendrogram, Orchidaceae, Dendrobium, Genetic diversity, Biology, Botany, Genetics

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Genetic Diversity of Dendrobium Species (Orchidaceae) Plants in China by ISSR — Research Paper | ScholarLens