Construction and Identification of Luciferase Reporter Recombinant Driven by FLT-1 Promoter

Objective:To evaluate FLT-1 gene promoter on gene transcription, we clone FLT-1 gene promoter and construct luciferase reporter recombinant regulated by FLT-1. Method:The FLT-1 gene promoter was amplified by polymerase chain reaction and subcloned into pGL3 Basic vector to construct pGL3 Basic luciferase reporter expression vector containing FLT-1 gene promoter(pGL3-FLT-Basic-luc). Then the expression vector was identified by restriction enzymes digestion,PCR analysis and DNA sequencing. Result:DNA sequencing and digestion confirmed that the recombinant of plamid pGL3-Basic-luc contained FLT-1 promoter identical to that in GeneBank. Conclusion:Luciferase reporter vectors regulated by FLT-1 promoter were successfully constructed, which will offer experimental foundation for gene therapy study of vascular disease.