Construction and Identification of Luciferase Reporter Recombinant Driven by Pit1 Promoter of Goose

To study the possible regulation mechanism of Pit1 gene expression,promoter sequence of Pit1 gene was subcloned and a Pit1 promoter-luciferase reporter vector was constructed.The promoter of Pit1 gene was cloned by genome walking and subcloned into the luciferase expression vector pGL3-Basic directly.The result showed that the recombinant reporter gene pGL3-Pit1 was constructed including correct target gene,and identified by restrictive endonuclease enzyme cutting,PCR and sequencing.The result indicate that the luciferase reporter gene eukaryotic expression vector containing Pit1 promoter sequences was constructed successfully,furthermore,the result will play an important role for analyzing the promoter activity and transcriptional regulation mechanism.