Construction of Luciferase Expression Vector Containing Mouse IL-10 Promoter

Objective To construct the luciferase expression vector containing mouse IL-10 promoter. Methods The fragment of IL-10 promoter was amplified from mouse genomic DNA by polymerase chain reaction(PCR).The amplified fragment was subsequently cloned into PGL3-basic.The recombinant plasmid was confirmed by restriction enzyme digestion and sequencing comparison. Results There were two fragments(4 800 bp and 682 bp) in electrophoresis after restriction enzyme digestion.The result of DNA sequencing showed that the sequence of the cloned IL-10 promoter was identical to that GeneBank had reported. Conclusions The luciferase expression vector containing mouse IL-10 promoter pGL3-IL10 is constructed successfully,and it will become the essential material for further study.