2012•Jilin yixueRequires access
The construction and identification of a luciferase reporter gene vector directed by human SOD_2 promoter
Lingzhi Zhong
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Abstract
Objective To clone the promoter of human superoxide dismutase 2 gene and insert it into a luciferase reporter gene vector,and then to identify and check the gene sequence.Method The promoter of the human SOD2 gene was amplified from human genomic DNA by PCR,which was cloned into pGL3-basic vector.Recombinant plasmid was cloned screening and amplified.The plasmid was determinded by double digestion reaction and DNA sequencing.Results The double digestion reaction and DNA sequencing showed that the sequence of cloned promoter was right and its gene locus was correct.Conclusion The cloning of the promoter of human SOD2 gene has been done successfully,and it will be a important basis for the following study of SP1 transcription factors interacting about vascular injury by Arsenic.