Study on genotoxicity effect of domestic sewage on cell of broad bean root tip
Yi Li
Abstract
Yi Li
Abstract
The paper investigated the genotoxicity effect of domestic sewage from different treatment processes on the cell of broad bean root tip.The micronucleus and chromosome aberration assay were conducted to determine the micronucleus rate and chromosome aberration rate of broad bean root tip cells induced by domestic sewage.The result indicated that domestic sewage could increase the micronucleus rate of the sells of broad bean root tip.Within certain concentration range of pollutant,the micronucleus rate increased with the increase of the concentration of pollutants,but beyond certain range,the micronucleus rate decreased with further increase of the concentration of pollutants.Domestic sewage could decrease the cell mitosis index.Furthermore,it also caused the increase of chromosome aberration rate.The conclusion is that domestic sewage has obvious genotoxicity effect on the cell of broad bean root tip.
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The paper investigated the genotoxicity effect of domestic sewage from different treatment processes on the cell of broad bean root tip.The micronucleus and chromosome aberration assay were conducted to determine the micronucleus rate and chromosome aberration rate of broad bean root tip cells induced by domestic sewage.The result indicated that domestic sewage could increase the micronucleus rate of the sells of broad bean root tip.Within certain concentration range of pollutant,the micronucleus rate increased with the increase of the concentration of pollutants,but beyond certain range,the micronucleus rate decreased with further increase of the concentration of pollutants.Domestic sewage could decrease the cell mitosis index.Furthermore,it also caused the increase of chromosome aberration rate.The conclusion is that domestic sewage has obvious genotoxicity effect on the cell of broad bean root tip.
Key concepts: Micronucleus, Genotoxicity, Root tip, Micronucleus test, Sewage, Chromosome aberration, Mitotic index, Pollutant