2009•Chinese Journal of Health Laboratory TechnologyRequires access
Research of rapid detecting microbial count in raw milk with ATP bioluminescence technique
Liu Na
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Abstract
Objective:To investigate the feasibility of ATP bioluminescence technique as a rapid detection method for the bacteria in crude milk.Methods:The quantity of ATP in bacterium was measured by bioluminescence technique.According to the relation of direct proportion between the quantity of ATP in bacterium and the quantity of bacterium,we can calculate total number of bacterial colony in crude milk.And the results were compared with those obtained by using standard plate counting method.Results:There was very good relativity between measuring results of ATP bioluminescence technique and standard plate counting method.Conclusion:The ATP bioluminescence technique for the quantitative measurement of bacteria in crude milk is of accurate and convenient feature and can be applied as a rapid detection method for crude milk bacteria.