Application of the ATP bioluminescence assay to screen bacterial contamination of cold dish

Objective To evaluate the feasibility of detecting bacterial contamination for cold dish by ATP bioluminescence assay,and to provide the scientific basis for food hygiene fast detection. Methods ATP bioluminescence assay and standard plate counting method were used respectively to detect 80 cold dish from 20 restaurants. Results Comparing with the standard plate counting method( cfu /g),the false positive rate of the ATP bioluminescence assay( cfu/g) was 18.8% and the false negative rate was 20.4%. Conclusion It was feasible for rapid preliminary test and evaluation of bacterial contamination by ATP bioluminescence assay at cold food institutions.